Tuesday, April 14, 2020

Cassia (Ketepeng Cina dan Senna)




HERBAL

MEDICINAL PLANT













Cassia
                                          Cassia alata (L) Roxb.
Cassia fistula L. (Leguminosae)
Cassia grandis L. f.  ++
Cassia angustifolia




by

RETTODWIKART THENU

Cassia alata (L) Roxb.

BOTANICAL DESCRIPTION (Ross, I. A. 2003)
This shrub of the LEGUMINOSAE family may grow up to about 3 meters tall. Leaves are pinnately compound, 30 to 40 cm long, with 6-12 pairs of broad oblong leaflets, blunt at the tip, unequal at the base, the terminal pair much larger, about 15 cm long and 8 cm wide. Flowers are roundish in compact axillary racemes, golden-yellow and very showy, about 20 to 30 cm long and 3-4 cm wide. The bracts are 2-3 by 1-2 cm. There are 5 unequal, oblong, 10-20 by 6-7 mm green sepals. The petals are bright yellow, ovate-orbicular to spathulate, shortclawed, 2 by 1-1.5 cm. There are 9-10 stamens; 2 large, 4 small, and 3-4 reduced. The anthers open via apical pores. There is only 1 pistil and glabrous ovary. Fruit are 4- winged pods, 10-15 cm long, dark brown when ripe. There are about 50 seeds, more or less quadrangular, arranged transversely in the pod.

ORIGIN AND DISTRIBUTION (Ross. I. A. 2003)
A native of tropical America, it is now widespread in warm countries. The plant grows in waste places, often along streams, banks, and in swamps.

TRADITIONAL MEDICINAL USES (Ross. I. A. 2003)
Australia. Hot water extract of dried leaves is taken orally as a catharticCAo61.
Bangladesh. Fresh leaves are squeezed and rubbed into ringwormCAOl6.
Brazil. Decoction of dried leaves is taken orally as an emmenagogue and abortifacientCAo63. Decoction of dried root is taken orally for malaria. Data were obtained by interviews with more than 8000 natives of various parts of BrazilcAOll.
Buka Island. Fresh leaves are squeezed
until soft and rubbed regularly onto the
affected part of the body to treat ringwormCA046.
Fiji. Hot water extract of dried leaves and stem is used externally for ringworm and skin diseasescAoss. The juice of the leaves and stem is squeezed out and rubbed on the affected area for ringworm and skin infectionscAo11, Infusion of dried leaves is taken orally as a blood purifier for worms and diarrheac Ao51.
Guatemala. Hot water extract of dried bark, leaves, and root is used externally for ringwormcAo34.
Guinea-Bissau. Hot water extract of root is taken orally as an emmenagogueCA002.
Guinea. A strong decoction of hot water extract of leaves is taken orally to promote abortion, and to treat leprosyCA034.
India. Fresh leaf juice is used for eczema. Juice from leaves is applied to affected area 3 times daily until curedcAos4. Fresh leaves are crushed and used for skin diseases, especially ringworm, eczema and scabiescAosl,cAolo. Leaf juice is used externally to treat leukoderma; a poultice of tender leaves is applied for over a monthCAosl.
Ivory Coast. Decoction of dried leaves is used externally to treat infections caused by dermatophytes, and orallycAol9 and externally to treat yeast infections caused by Candida albicans, as well as orally to treat bacterial infections caused by Escherichia colpol8.
Jamaica. Hot water extract of dried leaves is taken orally for diabetesCAo49.
Malaysia. Decoction of root is taken orally to ease stomachachecA028. Hot water extract of dried leaves is taken orally as a laxative; leaves are used externally against ringworm and scabies; the sap is used externally against external ulcersCA026 .
Mexico. Hot water extract of the plant is used externally as an astringent and against inflammation of rashes, orally as a purgative, anthelmintic and to relieve fevercAolO.
Nicaragua. Fresh leaves are used externally for ringworm and athlete's foot; decoction of the fresh leaves is taken orally for stomachache. It should not be given medicinally to pregnant women; it will induce abortioncAo3o.
Nigeria. Dried leaf, powdered with equal amounts of Piper guineense, is divided into small portions and taken orally with hot "Pap" to treat indigestion. Decoction of the dried leaves is taken orally to hasten delivery during labor; a strong decoction is taken orally to produce abortionCAol5. Decoction of dried leaves is used externally for ringworm, eczema and pustular skin infectionsCAOJ8. Infusion of dried leaves is taken orally as a purgativeCAo21. Fresh leaf juice is used externally to treat skin infectionscAo47. Leaf mixed with fruit pulp of Cucurbita pepo and Termitomyces microcarpus (mushroom) is taken orally to treat gonorrheacAo29. The ground inflorescence is mixed with "Pap" and taken orally to treat constipationcAol5.
Papau-New Guinea. Dried leaves are used externally for skin eruptions such as Tinea imbricata. Crushed leaves are rubbed on the skincA056 . Fresh leaves are used to treat grille, a skin fungus. Crushed leaves are rubbed into the skin affected by grilleCA024,CA025. Leaf juice is used externally for skin eruptions such as Tinea imbricata and ringwormCA052.
Philippines. Fresh leaves are used to treat fungal infection of the skin. The leaves are crushed and rubbed vigorously on the infected area of the skin CAOJ7.
Sierra Leone. Decoction of dried leaves is taken orally as a laxative CA05J.
Suriname. Fresh leaves are used externally for ringworm and skin diseasescAo56.
Tanzania. Decoction of leaves is taken orally as a purgativec Ao6o.
Thailand. Decoction of dried leaves is taken orally for asthmac A062j the hot water extract is taken orally as an antipyretic CA066 . Hot water extract of dried entire plant is taken orally as a catharticCAo68. Pulverized flower is taken orally for asthmac Ao62. Hot water extract of dried seeds is taken orally as an anthelminticCAo68.
Trinidad. Seeds and leaves are used as anthelmntics cM77 .
West Africa. Hot water extract of dried leaves is taken orally as an ecbolic and emmenagogueCA044. Hot water extract of fresh leaf juice is used for parasitic skin diseases CAOJI. Strong decoction of hot water extract of leaves is taken orally as an abortifacient CAool. Water extract of the leaf is used to treat bacterial infections caused by Escherichia coli and fungal infections caused by Candida albicans and dermatophytes CA074.
West Indies. Hot water extract of flowers is used externally as an antibacterialcAo41. Leaf teas are used for intestinal worms CA04J. Seeds are taken orally as a vermifuge CAo43.

CHEMICAL CONSTITUENTS (Ross. I. A. 2003)
(ppm unless otherwise indicated)
Alatonal: StCA009
Aloe emodin: PICA012
Alquinone: Rt 10cAoo8
Anthraqu inone, 1-5-dihydroxy-2-methyl: StCA027
Anthraquinone,5-hydroxy-2-methyl 1-0-rutinoside: StCA027
Benzoquinone, 2-6-dimethoxy: StCA007
Beta sitosterol: RtCA027
Chrysarobin: Lf CAOOS
Chrysophanol: PICA004
Chrysophanol glycoside: LfCAOOS
Chrysophanic acid: LfCA012
Chrysoeriol-7 -O(2-0-beta-Dmannopyranosyl)-beta D-allopyranoside: SdCA006
Dalbergin: StCA007
Daucosterol: StCA007
Deoxycoeluatin: LfCAOll
Emodin: St 3.3CA020,CA007
Kaempferol: LfCA017
Luteolin: StCA007
Phytosterol: Lf, St BkcA017
Rhamnetin-3-0-(2-0-beta-Dmannopyranosyl)-beta-D-allopyranoside:SdCA072
Rhein: PICA004
Rhein glycoside: LfCA003
Santal: StCA007
Tannin: LfCAOOS

PHARMACOLOGICAL ACTIVITIES AND CLINICAL TRIALS
(Ross, I. A. 2003)
Abortifacient effect. Ethanol/water (50%) extract of dried leaves, administered by gastric intubation to rats at a dose of 125.0 mg/ kg, was inactiveCA06J.
Analgesic activity. Ethanol (85%) extract of dried leaves, administered intraperitoneally to mice at a dose of 100.0 mcg/kg, was activeCA058. Ethanol/water (1:1) extract of aerial parts, administered intraperitoneally to mice at a dose of 500.0 mg/kg, was inactive vs tail pressure methodCAo64. Leaf extract, administered intraperitoneally to mice and rats, was active using tail clip, tail flick, tail immersion, and acetic acid-induced
writhing methods. Maximum analgesic activity was apparent 2 hours after injection of the extract. Fifty mg of kaempferol 3-0-sophoroside appeared equivalent to 100 mg of the extractCA073.
Antibacterial activity. Chloroform extract of dried leaves, at a concentration of 5.0 mcg/ml on agar plate, was active on Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli, Micrococcus luteus, and Staphylococcus aureusCAOJ8. The chromatographic fraction, undiluted on agar plate, was active on several Gram positive and Gram negative organismsCA04J. The acetic acid extract of dried leaves, at a concentration of 5.0 mg/ml, was active on Bacillus subtilis, Escherichia coli, Micrococcus luteus, Pseudomonas aeruginosa, and Staphylococcus aureusCAOJ8. Chloroform extract of dried stem bark, at a concentration of 1.0 mg/disk on agar plate, was active on Bacillus cereus, Bacillus subtilis, Pseudomonas aeruginosa, Salmonella paratyphi B, Salmonella typhi, Shigella dysenteriae, Shigella flexneri, Shigella sonnei and Staphylococcus aureus. It was inactive on Aeromonas hydrophilia, Escherichia coli, Salmonella paratyphi A, Vibrio cholera, Vibrio mimicus and Vibrio parahemolyticus. The methanol extract was active on Bacillus cereus, Bacillus subtilis, Escherichia coli, Salmonella paratyphi B, Salmonella typhi, Shigella flexneri, Shigella sonnei and Vibrio cholera, and inactive on Aeromonas hydrophilia, Pseudomonas aeruginosa, Salmonella paratyphi A, Vibrio mimicus and Vibrio parahemolyticus.
The petroleum ether extract was active on Salmonella paratyphi B and Shigella flexneri, and inactive on Aeromonas hydrophilia, Bacillus cereus, Bacillus subtilis, Escherichia coli, Salmonella paratyphi A, Salmonella typhi, Vibrio cholera, Vibrio mimicus, and Vibrio parahemolyticus; active on Shigella sonnei at a concentration of 1.4 mg/disk, and Shigella dysenteriae and Staphylococcus aureus, MIC 0.8 mg/diskCA01J. Ethanol (85%) extract of dried leaves, at a concentration of 10.0% on agar plate, was active on Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, and Staphylococcus aureusCAOJ5 . Methanol extract of the dried leaves, at a concentration of 1.0 mg/disk on agar plate, was active on Bacillus subtilis, Escherichia coli, Salmonella paratyphi B, Shigella flexneri, Shigella sonnei, and Vibrio cholera, and inactive on Aeromonas hydrophilia, Bacillus cereus, Pseudomonas aeruginosa, Salmonella paratyphi A, Salmonella typhi, Vibrio mimicus, and Vibrio parahemolyticus. The methanol extract of dried leaves, on agar plate, showed MIC 0.2 mg/disk for Shigella dysenteriae and 0.4 mg/ disk for Staphylococcus aureus. Petroleum ether extract of dried leaves, at a concentration of 1.0 mg/disk on agar plate, was active on Salmonella paratyphi B, Shigella flexneri, and Shigella sonnei, and inactive on Aeromonas hydrophilia, Bacillus cereus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Salmonella paratyphi A, Salmonella typhi, Staphylococcus aureus, Vibrio cholera, Vibrio mimicus, Vibrio parahemolyticus, and Shigella dysenteriaeCA01J. Ethanol (95%) extract of dried leaves, at a concentration of 100.0 mg/disk (expressed as dry weight of plant) on agar plate, was active on Bacillus subtilis, and inactive on Escherichia coli, Salmonella typhosa, Shigella dysenteriae, and Staphylococcus aureus. Water extract, at a concentration of 20.0 mg/disk, was inactive on Bacillus subtilis, Escherichia coli, Salmonella typhosa, Shigella dysenteriae, and Staphylococcus aureusCA040. Ethanol (95%) extract of dried leaves, at a concentration of 500.0 mg/ml on agar plate, was inactive on Escherichia coli, Proteus mirabilis, Proteus vulgaris, and Staphylococcus epidermidis. A concentration of 500.0 micromols/ml was inactive on Staphylococcus aureusCA026. A concentration of 5.0 mg/ml was active on Bacillus subtilis, Escherichia coli, Micrococcus luteus, Pseudomonas aeruginosa and Staphylococcus aureusCA038.  Ethanol (95%) extract ofleaves, on agar plate, was active on Bacillus subtilis, Escherichia coli, Klebsiella pneumonia, Serratia marcescens, and Staphylococcus aureusCA045. Ethanol/water (1: 1) extract of aerial parts, at a concentration of 25.0 mcg/ml on agar plate, was inactive on Bacillus subtilis, Escherichia coli, Salmonella typhosa, Staphylococcus aureus and Agrobacterium tumefaciensCA064. Water extract of dried leaves, at variable concentrations, was active on Pseudomonas aeruginosa and Staphylococcus aureusCAOl4. The water extract of dried leaves, on agar plate, was active on Escherichia coli, LCso 1.0 mg/unit and MIC 1.6 mg/ mL cAo19,cAo18. The methanol extract of leaves, flowers, stem, and root bark produced a broad spectrum of antibacterial activity. The activity was increased on fractionation with petrol, dichloromethane and ethyl acetate. The dichloromethane fraction of the flower extract being the most effectivecAo76. Methanol extract of leaves, flowers, stem, and root barks produced a broad spectrum activity. The activity was increased on fractionation with petrol, dichloromethane, ethyl acetate. The dichloromethane fraction of the flower extract was the most effectivecAo78.
Anticlastogenic activity. Juice of leaves, administered by gastric intubation to mice at a dose of 25.0 ml/kg, was active on bone marrow cells vs mitomycin C-, dimethylnitrosamine-, and tetracycline-induced micronucleic Ao22.
Anticonvulsant activity. Ethanol/water (1: 1) extract of aerial parts, administered intraperitoneally to mice at a dose of 500.0 mg/kg, was inactive vs electroshockinduced convulsionscAo64.
Antifungal activity. Chloroform, acetic acid and ethanol (95%) extracts of dried leaves, at concentrations of 5.0 mg/ml on agar plate, showed weak activity on Aspergillus fumigatus, Lasiodiplodia theobromae, Penicillium italicum and Trichophyton mentagrophytesCAo38. Dried leaf, at a concentration of 20.0% on agar plate, was inactive on Aspergillus flavus, Aspergillus fumigatus, Mucor species, Penicillium species, and Rhizopus species. Water extract of dried leaves, at concentrations of 80.0%, 90.0%, and 100.0% applied externally on human adults, was active on Malassezia furfur. The extract was applied to the neck, hands, and trunk. Pityriasis versicolor was treatedCAo69. Methanol (85%) extract of dried leaves, at a concentration of 2.5% on agar plate, was active on Microsporum gypseum, Trichophyton mentagrophytes, and Trichophyton rubrumCA059. Ethanol (95%) extract of dried leaves, at a concentration of 500 mg/ml on agar plate, was active on Microsporum canis, Microsporum gypseum, Trichophyton mentagrophytes, and Trichophyton rubrum, and weakly active on Aspergillus niger, Cladosporium werneckii, Fusarium solani, and Penicillium species, and inactive on Candida albicans, Rhodotorula rubra, and Saccharomyces cerevisiaeCA026. Ethanol/water (1: 1) extract of aerial parts, at a concentration of 25.0 mcg/ml, was inactive on Microsporum canis, Trichophyton mentagrophytes, and Aspergillus nigerCAo64. Hot water extract of dried bark, leaf and root, at a concentration of 1.0 ml in broth culture, was inactive on Epidermophyton floccosum, Microsporum canis, Microsporum gypseum, Trichophyton mentagrophytes vars. Algodonosa and Granulare, and Trichophyton rubrumCAOJ4. Juice of the dried entire plant, on agar plate, was inactive on Epidermophyton floccosum, Microsporum gypseum and Trichophyton rubrumCA041. Hot water extract of dried leaves, at a concentration of 5.0% on agar plate, was active on Trichophyton mentagrophytesCAo48.
Antihistamine activity. Ethanol/water (1: 1) extract of dried leaves, at variable concentrations, was active on guinea pigileum cAo66.
Antihyperglycemic activity. Petroleum ether extract of shade-dried leaves, administered by gastric intubation at a dose of 100.0 mg/kg to rats, was active vs streptozotocin-induced hyperglycemiacAol7.
Anti-inflammatory activity. Ethanol (85%) extract of dried leaves, administered intraperitoneally to mice at a dose of 100.0 mg/kg, was active vs carrageenin-induced pedal edema and cotton pellet granulomacAo39. Ethanol/water (1: 1) extract of aerial parts, administered orally to rats at a dose of 500.0 mg/kg, was active vs carrageenin-induced pedal edema. Animals were dosed 1 hour before carrageenin injectionscAo64. Shade-dried leaves, administered by gastric intubation to rats at dose of 150.0 mg/kg, were activeCA033.
Antimutagenic activity. Methanolinsoluble fraction of dried flowers was active vs methylnitrosamine, methyl methanesul-fonate, or tetracycline-induced genotoxicity CA023.
Antipyretic activity. Ethanol/water (1:1) extract of dried leaves, administered by gastric intubation at variable concentrations to rabbit, was inactive vs yeastinduced pyrexiacAo66.
Antispasmodic activity. Ethanol/ water (1: 1) extract of dried leaves, at variable concentrations, was active on guinea pigileumCA066. Ethanol/water (1: 1) extract of aerial parts was inactive on guinea pig ileum vs ACh- and histamine-induced spasmsCA064.
Antitumor activity. Acid/water, ethanol (95%) and water extracts of dried leaves, administered subcutaneously to mice of both sexes at doses of 0.02 gm/kg, showed weak activity on Sarcoma 37cA067.
Antiyeast activity. Chloroform, acetic acid, and ethanol (95%) extracts of dried leaves, at concentrations of 5.0 mg/ml on agar plate, showed weak activity on Candida albicanscA038. Dried leaf, at a concentration of 20.0% on agar plate, was inactive on Candida albicanscAol9. Ethanol (95%) extract of dried leaves, at concentrations of 20.0 and 100.0 mg/disk on agar plate, were inactive on Candida albicanscAo4o. Ethanol/water (1: 1 ) extract of aerial parts, at a concentration of 25.0 mcg/ml on agar plate, was inactive on Candida albicans and Cryptococcus neoformansCA064•  Juice of the dried entire plant, on agar plate, was inactive on Candida albicans, Cryptococcus neoformans, and Saccharomyces cerevisiae CA041 . Water extract of dried leaves, on agar plate, showed lClo 28.0 mg/ml and MlC 0.39 mg/ml on Candida albicansCA019,CA018.
Barbiturate potentiation. Ethanol/ water ( 1: 1) extract of aerial parts, administered intraperitoneally to mice at a dose of 500.0 mg/kg, was inactiveCA064.
Choleretic effect. Leaf extract, administered orally to rats at doses of 15,30, and 60 mg/kg, was active. Choleretic activity at the 15 mg/kg dose level was better that a group treated with 15 mg/kg of hydroxycyclohexenyl butyrate, a synthetic choleretic. At elevated doses, the extract tends to inhibit bile secretionCA074.
Diuretic activity. Ethanol/water (1: 1) extract of aerial parts, administered intraperitoneally to male rats at a dose of 250.0 mg/kg, was active. Urine was collected for 4 hours posttreatment from saline-loaded animalscAo64.
Embryotoxic effect. Ethanol/water (50%) extract of dried leaves, administered by gastric intubation to rats at a dose of 125.0 mg/ kg, was inactivecA063.
Estrous cycle disruption effect. Ethanol/ water (50%) extract of dried leaves, administered by gastric intubation to rats at a dose of 125.0 mg/kg, was equivocalcAo63.
Hypoglycemic activity. Ethanol/ water (1: 1) extract of aerial parts, administered orally to rats at a dose of 250.0 mg/kg, was inactive. Less than 30% drop in blood sugar level was observedcAo64. Hot water extract of dried leaves, administered by gastric intubation to dogs at a dose of 200.0 ml/ animal, produced weak activityCAo49. Petroleum ether extract of shade-dried leaves, administered by gastric intubation to rats at a dose of 400.0 mg/kg, was inactiveCAO\7. Leaf extract, administered orally to streptozotocin-induced hyperglycemic rats, reduced the blood sugar value in streptozotocin-induced hyperglycemic rats. The extract had no effect on glucose levels in normoglycemic animalscAo71.
Hypotensive activity. Ethanol/ water (1: 1) extract of dried leaves, administered intravenously to dogs at variable dosages, was inactiveCA066.
Hypothermic activity. Ethanol/ water (1: 1) extract of aerial parts, administered intraperitoneally to mice at a dose of 500.0 mg/kg, was inactivecAo64.
Laxative effect. Ethanol/water (1: 1) extract of dried leaves, administered orally at variable dosages to human adults, was active. Patients with at least 72 hours of constipation were treated with either placebo or Cassia. Out of 24 patients treated with Cassia, 83% passed stools in 24 hours. The success rate in the placebo group was only 18%CA012. Hot water extract of dried leaves, administered by gastric intubation at a dose of 500.0 mg/kg to rats, was active. The extract had 70% of the activity of senna, Cassia acutifoliaCAOJ6. The infusion, at a dose of 800.0 mg/kg, was also activeCA021. Leaves, administered orally to male albino rats, were active. The leaves of Cassia acutifolia Del. was used as reference standardcAo70.
Molluscicidal activity. Ethanol (95%) and water extracts of dried trunk bark, at concentrations of 10,000 ppm, were inactive on Biomphalaria glabrata and Biomphalaria stramineaCA065.
Pityriasis versicolor effect. Leaf extract has been effective in a 1 O-year human study with no side effectCA07j..
Semen coagulation effect. Ethanol/water (1: 1) extract of aerial parts, at a concentration of 2.0%, was inactive on rat semenCA064.
Spermicidal effect. Ethanol/water (1: 1) extract of aerial parts, at a concentration of 2.0%, was inactive on rat spermCA064.
Toxic effect (general). Ethanol (85%) extract of dried leaves, administered intraperitoneally to mice at a dose of 2.0 gm/kg, was inactiveCAOS8,CA039. Ethanol/water (1: 1) extract of dried leaves, administered by gastric intubation and subcutaneously at doses of 10.0 gm/kg to mice, was inactivecAo42.
Toxicity assessment (quantitative). Ethanol/ water (1: 1) extract of aerial parts, administered intraperitoneally to mice, showed LDso 1.0 gm/kgCA064.
Wound healing acceleration. Petrol (gasoline) extract of dried leaves, applied externally to rabbits at a dose of 10.0%, was active. The extract, in the form of a polyethylene glycol ointment, was applied daily to a skin wound that had been inoculated with Staphylococcus aureus or Pseudomonas aeruginosa. By 21 days, area of wound was 87.6% healed over vs 56.2% on controlscAo61.

BENEFITS DATA (Permenkes No.6. 2016)
Preclinical Test:
Methanol extract, water, alkaloid salts and alkaloid base of C. alata leaves showed very strong and significant activity against 2 microbes (Dermatophylus congoensis, Actinomyces bovis and 5 fungi, Microsporum canis, Blastomyces dermatitidis, Trichophyton mentagrophytes, Candida albicans, Aspergillus flav. the strongest was observed in methanol extracts, this result supports the use of C. alata extract as an antifungal in the skin.
 Clinical Test:
RCT research on 33 patients in prison (19 studies and 14 controls) with skin infections of Tineasis versicolor and Tinea corporis (microscopic visible fungal infections of Epidermophyton floccusum and Cryptococcus sp) were asked to bathe and rub soap (leaf powder C. alata, NaOH, and oil) coconut 1.5% ww) on diseased skin twice a day for 1 month. The results showed that 16 patients (94.1%) who were given C. alata soap significantly lost their skin infection, while the control group had no change. The results confirm the use of C. alata as a therapy for dermatitis due to fungi. The therapeutic effect of C. alata leaf extract on Pityriasis versicolor was reported from a 10-year human study which showed that leaf extract was effective for the treatment of Pityriasis versicolor.

DOSAGE (Permenkes No.6. 2016)
Posology
Ointment: 2 x 1 / day
Soap: 2 x 1 / day

SIDE EFFECTS (Permenkes No.6. 2016)
A 10-year study on the treatment of fungal dermatitis on the skin in India reported no side effects.

Cassia fistula L.
(Leguminosae)
Golden Shower Tree, Indian Laburnum, Purging Cassia

DESCRIPTION:
Cassia fistula L. is a large tree, which grows to 10 m tall. Leaves are alternate, pinnate, 3–8 pairs of leaflets, broadly ovate and pointed. Flowers are dense, bright yellow and about 4–5 cm across. Fruit pods are long, 30–60 cm, cylindrical, brown in colour and contains many seed.[1–3]
Medicinal Parts: The medicinal parts of the plant are the bark, fruit and seeds.
Flower and Fruit: The flowers are in loose, hanging, 30 to 50 cm long racemes. There are 5 pale yellow, ovate petals. The diameter of the corolla is approximately 3.8 cm. The calyx is deeply divided and 5-toothed. There are 10 stamens. The fruit is a legume, 30 to 60 cm long, hanging and indehiscent.
Leaves, Stem and Root: Cassia fistula is a tree, that grows up to 9 m high. The leaves are 20 to 40 cm long, 4- to 8-paired pinnate. The leaf spindle is hairy and the leaflet is petiolate, ovate to oval, acuminate, 5 to 12 cm long, 4 to 9 cm wide and silvery haired underneath. The young bark is smooth and greenish-gray. Older bark is dark brown and rough.
Habitat: India, Africa and South America
Production: Cassia pods are the dried ripe fruit of Cassia fistula.
Not to be Confused With: Very occasionally the tree has been confused with South American Cassia species.
Other Names: Canafistula. Indian Laburnum, Pudding Pipe Tree, Purging Cassia

ORIGIN:
Native to India and Sri Lanka.[2,4]

PHYTOCONSTITUENTS:
Fistucacidin, chrysophanic acid, chrysophanol, clitorin, sennosides A and B, chrysophanein and others.[2,4–9]
Anthracene derivatives (1% in the mesocarp): sennosides, fistulinic acid
Monosaccharides/oligosaccharides (50%): particularly saccharose
Fruit acids: citric acid
Steroids: sterols (in the seeds), including beta-sitosterol
Fatty oil (in the seeds)

TRADITIONAL MEDICINAL USES:
The whole plant is used for anthrax, burns, cancer, constipation, convulsions, delirium, diarrhoea, dysentery, dysuria, epilepsy, fever, influenza, gravel, haematuria, pimples, syphilis, tumours and worms.[4,10]
The leaf is used for skin diseases (juice), healing ulcers, for ringworm and irritation of skin (juice of young leaves), facial paralysis and rheumatism (paste).[10] The raw black pulp found between the seeds is a popular remedy for constipation.[3,4] It is also used as a cathartic, for rheumatism and snakebite (pulp), treats bacterial infections (pulp mixed with leaves of Cassia angustifolia), liver complaints, heart disease, reduce fever, as abortifacient, demulcent and is useful in liver, throat, eye diseases, convulsions and sores.
The seed is an emetic, carminative, appetiser, and is used for constipation, jaundice, cancer on face and syphilis.[10]
The roots act as a purgative while the rootbark is used for cleansing wounds.[4] The root is also used as an astringent, tonic, febrifuge, for skin diseases, leprosy, tuberculous glands, syphilis and epilepsy.[10]

Pharmacological Activities:
Antimicrobial,[11–14] Anticholinergic,[15] Antifertility,[16] Anti-inflammatory,[17] Antineoplastic,[18] Antioxidant,[19–22] Depressant,[23] Hepatoprotective,[24–26] Hypocholesterolaemic,[27] Antileishmanial,[28] Larvicidal[29] and Wound healing.[30,31]
Abortifacient (f; ADP; JFM); Alexiteric (f; WO2); Allergenic (1; VOD); Amebicide (1; JAC7:405); Analgesic (1; JAF50:5042; MPI; WO2); Anthelmintic (f; WO2); Antidiabetic (f; X16242721); Antiinflammatory (f1; WO2); Antioxidant (1; JAF50:5042; X12188605; X15652272; X15991578); Antiperiodic (f; SKJ; WOI); Antiradicular (1; JAF50:5042); Antisecretory (f; WO3); Antiseptic (1; PH2; X16678369); Antitumor (f1; ADP; X16242721); Antitussive (1; JAF50:5042); Antiviral (1; ADP; PH2; WO2); Aperient (f; EFS); Astringent (f; EGG; SKJ; WO2); Bactericide (1; JAF50:5042; MPG; X12608640; X16678369); Demulcent (f; ADP); Dentifrice (f; WO2); Deobstruent (f; DEP); Emetic (f; KAB; WO2); Febrifuge (f; DEP; SKJ; SUW); Fungicide (1; WO2); Hemagglutinant (1; MPG); Hepatoprotective (f1; X15991578; X16242721); Hypocholesterolemic (1; JAC7:405); Hypoglycemic (1; ADP; JAC7:405; MPG; WO2); Immunostimulant (1; MPG); Interferonigenic (1; MPG); Laxative (f1; GHA; HHB; MPG; NPM; PH2; VOD); Orexigenic (f; KAB; NPM); Polygalacturinase-Inhibitor (1; MPG); Protopectinase Inhibitor (1; MPG); Purgative (f1; ADP; EGG; JAF50:5042; NPM); Refrigerant (f; WO2); Stomachic (f; WO2); Tonic (f; NPM; SKJ; WO2); Uterotonic (1; WO3); Vermifuge (f; VOD); Vulnerary (f; X16242721).

Indications:
Abscesses (f; WO2); Acne (f; ADP); Adenopathy (f; JLH; SKJ); Amebiasis (1; JAC7:405; WO2); Amenorrhea (f; ADP); Anorexia (f; KAB; PH2); Anthrax (f; WO2); Arthritis (f; SKJ; WO3); Asthma (f; NPM; WO2); Bacillus (1; MPG); Bacteria (f1; JAF50:5042; SKJ; X12608640; X16678369); Biliousness (f; NAD; SKJ); Bites (f; ADP; NAD); Bleeding (f; WO2); Blindness (f; ADP); Blood (f; WO3); Boils (f; WO3); Burns (f; ADP); Cancer (f1; ADP; JLH; MPG); Cancer, abdomen (f; JLH); Cancer, colon (f; JLH); Cancer, face (f; SKJ); Cancer, gland (f; JLH); Cancer, liver (f; JLH); Cancer, throat (f; JLH); Cancer, uterus (f; JLH); Cardiopathy (f;
ADP; NAD; WO3); Cerebrosis (f; DEP; WO2); Chilblains (f; ADP); Cholecocystosis (f; JFM); Colic (f; NAD); Conjunctivosis (f; WO2); Constipation (f1; ADP; AHL; DEP; GHA; HHB; PH2); Convulsions (f; ADP); Coughs (1; JAF50:5042; WO2); Delirium (f; ADP); Dermatosis (f; ADP; NPM; PH2; VOD; WO2); Diabetes (f1; ADP; JAC7:405; NPM; WO2); Diarrhea (f; NPM); Diphtheria (1; WO2); Dysentery (f; NPM; WO2; WO3); Dyspepsia (f; GHA); Dysuria (f; ADP; WO2); Eczema (f; NPM); Enterosis (f; WO2); Epilepsy (f; ADP; SKJ); Escherichia (1; MPG; WO3); Fever (f; ADP; PH2; SUW); Flu (f; AHL); Fracture (f; WO2); Fungus (1; WO2); Gas (f; NAD; PH2; VOD); Gastrosis (f; GHA; WO2); Giddiness (f; NPM); Gonorrhea (f; WO2); Gout (f; DEP; VOD; WO2); Gravel (f; ADP); Hematemesis (f; JAF50:5042); Hematuria (f; ADP); Hemorrhoids (f; GHA); Hepatosis (f1; ADP; JLH; X16242721); Herpes (f; WO2); High Cholesterol (1; JAC7:405); High Triglycerides (1; JAC7:405); HIV (f; VOD); Hyperglycemia (1; JAC7:405; WO2); Impostume (f; JLH); Induration (f; JLH); Infection (f1; ADP; PH2; X16242721; X16678369); Inflammation (f1; JLH; VOD; WO2); Itch (f; PH2; WO2); Jaundice (f; ADP; PH2); Leprosy (f; WO2); Leukoderma (1; JAF50:5042); Malaria (f; SKJ); Migraine (f; WO3); Mycosis (f1; ADP; JAF50:5042); Nausea (f; SUW); Ophthalmia (f; ADP; WO2); Pain (f1; WO3; JAF50:5042; MPI; WO2); Paralysis (f; DEP; NAD; SKJ); Parasites (f; VOD); Pharyngosis (f; WO2); Pregnancy (f; VOD); Prickly Heat (f; JFM); Prurigo (f; WO2); Psoriasis (f; ADP); Pulmonosis (f; ADP; IED); Pustule (f; DEP); Pyoderma (f; ADP); Respirosis (f; IED); Rheumatism (f; DEP; SKJ; VOD; WO2); Ringworm (f; ADP; DEP); Salmonella (1; WO2); Sarcoma (1; MPG); Scabies (f; ADP); Snake Bite (f; NPM; SKJ; SUW); Sores (f; WO2); Sore Throat (f; NPM; WO2); Staphylococcus (1; MPG); Stings (f; JFM); Stomachache (f; WO2); Swelling (f; JLH; WO3); Syphilis (f; ADP; NPM); Tonsillitis (f; WO3); Toothache (f; NPM); Tuberculosis (f; SKJ; VOD); Tumors (f1; JLH; MPG); Typhus (1; WO2); Ulcers (f; GHA); Vaccinia (1; MPG); VD (f; ADP; NPM; WO2); Viruses (1; MPG;PH2); Wet Dream (spermatorrhea, nocturnal emissions) (f; WO2); Worms (f; VOD; WO2); Wounds (f; X16242721).

DOSAGES:
Mode of Administration: Whole drug preparations are for internal use.
Preparation: To prepare an extract, use pulp and distilled water in a 1:1 ratio, macerate, then exhaustively percolate with distilled water and filter. Evaporate to a soft extract.

FNFF = !! Flowers, leaves, and fruit pulp eaten, the latter possibly purgative (FAC). Seed eaten (TAN).
4–8 g fruit pulp (HHB; PH2). Asian Indians use the plant in clarified butter for g • landular tumors (JFM).
·         Curacaoans take the leaf decoction for gallbladder problems (JFM).
·         Dominicans suggest a floral syrup for constipation and flu (AHL).
·         Haitians take salted leaf or fruit decoction for worms (VOD).
·         Jharkandi natives take ca. 5 g endosperm with honey 2–3 mornings for diabetes (ADP).
·         Nepalese take 4 tsp fruit pulp 3×/day for hematuria, diarrhea, and dysentery (NPM).
·         CNepalese take 6 tsp teaspoons pulp paste 4×/• day for giddiness (NPM).
·         Oriyan women insert leaf paste into genitals once daily for a week for amenorrhea (ADP).
·         Punjabi use root as febrifuge and tonic (DEP).
·         Rhodesians use the plant for anthrax, blackwater fever, blood poisoning, dysentery, and malaria (KAB).
·         Yunani consider leaves antiinflammatory, flowers purgative, the fruits abortifacient, demulcent, febrifuge, and purgative, using for chest, eye, liver, rheumatic, and throat complaints (KAB).
OVERDOSAGE
In the case of overdose, cramp-like gastrointestinal complaints could occur as a side effect of the laxative effect of the drug. Prolonged administration leads to loss of electrolytes, particularly of potassium ions, which in turn leads to aldosteronism, albuminuria, hematuria, inhibition of intestinal motility, muscle weakness, enhancement of the effect of cardioactive steroids and an influence upon the effect of antiarrhythmics. In rare cases, administration of the drug may lead to cardiac arrhythmia, nephropathy, edema and accelerated osteoclasis.

EXTRACTS:
Oil from the pod was active against Klebsiella at 500 ppm (FT67(2):173). Pods have highest total phenolic, proanthocyanidin, and flavonoid contents and antioxidant potentials (TEAC = 992 +/– 0.4 μM/g dry weight; FRAP = 811 +/- 23 μM/g dry weight) (X12188605).

Adverse Reactions: No information as yet.

Toxicity: In cases of overdose or prolonged administration, loss of electrolytes, especially potassium ions, aldosteronism, albuminuria, haematuria, inhibition of intestinal motility and muscle weakness may occur. Rarely, cardiac arrhythmia, nephropathy, oedema, and accelerated osteoclasis may arise.[32]

Contraindications: Contraindicated with acute inflammatory diseases of intestine and appendicitis. Should not be used during pregnancy and while nursing. Should not be used in children under 12 years of age.[32]

Drug-Herb Interactions: Interaction with anthranoid laxatives.[33] Enhancement of effects of cardioactive steroids may occur. Effects of antiarrhythmics may also be affected.[32]



----------------------------------------‘’’’’’’’’’’’’’’’’’’’’’’’’’’’’------------------------------------------
SENNA
Cassia species


TRADE NAMES
Senna Extract, Senna Leaf, Ex-Lax Regular Strength, Ex- * Lax Maximum Strength, X-Prep Bowel Evacuant Liquid, SenokotXTRA, Senokot, Senokot Children's Syrup

DESCRIPTION
Medicinal Parts: The medicinal parts are the leaves, fruit and flowers.
Flower and Fruit: The flowers are yellow, occasionally white or pink. They are located in axillary or terminal positions on erect racemes. The calyx is deeply divided with a short tube and 5 regular, imbricate sepals. There are 5 layered petals. The 4 to 10 stamens are often irregular and partially sterile. The ovary is sessile or short-stemmed with a short or oblong style. The pod can be cylindrical or flat, angular or winged and often with horizontal walls between the seeds. The seeds are numerous and either horizontally or vertically compressed.
Leaves, Stem and Root: The genus Cassia comprises shrubs, subshrubs, and herbaceous perennials with paired-pinnate <J leaves. There are axes with stem glands either between the leaflets or on the petiole. The stipules have varying shapes.
Habitat: Cassia species is found in the tropical and subtropical regions of all continents except Europe. Most varieties are indigenous to North, Central, and South America.
Other Names: Tinnevelly Senna, India Senna, Alexandrian Senna, Khartoum Senna

PLANT MATERIAL OF INTEREST: LEAFLETS
General appearance
Macroscopically, the leaflets are lanceolate or lanceolate-ovate, unequal at the base, with entire margin, acute-mucronate apex and short, stout petioles; sometimes broken; 1.5–5cm in length and 0.5–1.5cm in width, bearing a fine pubescence of appressed hairs, more numerous on the lower surface (17).
Organoleptic properties
The colour is weak yellow to pale olive (1, 2). The odour is characteristic, and the taste is mucilage-like and then slightly bitter (1, 3).
Microscopic characteristics
Epidermis with polygonal cells containing mucilage; unicellular thick-walled trichomes, length, up to 260μm, slightly curved at the base, warty; paracytic stomata on both surfaces; under the epidermal cells a single row of palisade layer; cluster crystals of calcium oxalate distributed throughout the lacunose tissue; on the adaxial surface, sclerenchymatous fibres and a gutter-shaped group of similar fibres on the abaxial side containing prismatic crystals of calcium oxalate (1).
Powdered plant material
Light green to greenish yellow. Polygonal epidermal cells showing paracytic stomata. Unicellular trichomes, conical in shape, with warty walls, isolated or attached to fragments of epidermis. Fragments of fibrovascular bundles with a crystal sheath containing calcium oxalate prisms. Cluster crystals isolated or in fragments of parenchyma (2, 3).

HOW TO USE SENNA
Most senna preparations are standardized to contain a predictable quantity of sennosides. In Sennokot, each tablet delivers 8.6 milligrams of sennosides, with the usual dosage ranging from two to four tablets at a time. Other preparations usually provide a specific amount of dried senna leaves or pods, ranging from 150 to 450 milligrams. Standard doses are more difficult to get in herbal teas containing senna. If you prefer to use a tea, become familiar with a specific brand, and steep it a consistent amount of time to ensure a reproducible response.
AYURVEDA AND SENNA
Senna is considered a laxative of moderate potency. With its bitter and cooling effects on the physiology, it is pacifying to Pitta and Kapha. Used excessively, it can be depleting and aggravating to Vata. According to Ayurveda, the digestive tract should be prepared before taking senna by eating lightly and increasing your intake of oily foods such as sesame seeds or ghee (clarified butter). This “oleation” is said to ensure a smoother, more comfortable elimination in response to the herbal laxative.

ACTIONS AND PHARMACOLOGY COMPOUNDS
Anthracene derivatives (2.5-3.5%): chief components sennosides A, Al and B, as well as sennosides C and D
Naphthacene derivatives: including 6-hydroxymusizin glucoside (0.85% in Cassia senna), tinnevellin-6-glucosides (0.3% in Cassia angustifolia)
Alexandrian and Indian senna leaves have similar chemical compositions, especially in their anthracene derivatives. They contain as their active constituents dianthrone glucosides (usually 1.5–3.0%), consisting mostly of sennosides A and B (rhein-dianthrone glucosides), with minor amounts of sennosides C and D (rhein-aloe-emodin-heterodianthrone glucosides) and aloe-emodin dianthrone glucoside also present.4–10 These dianthrone glycosides are reportedly absent in fresh leaves, and it appears that they are formed during the drying process through enzymatic oxidation of monoanthrone glycosides that are present in fresh leaves but normally absent in dried leaves (also see cascara).5,11
There is also evidence of the existence of primary glycosides of the sennosides (with additional sugar molecules) that are more active than the sennosides.6 Senna leaves also contain small amounts of free anthraquinones (rhein, aloe-emodin, chrysophanol, etc.) and theirO-glycosides and C-glycosides.2,5–8,11,12 Alexandrian senna leaves generally have a higher sennoside content than Indian senna leaves. Two benzophenone glucosides have recently been isolated from Tinnevelly senna pods and were characterized as 60-carboxy-20, 6-dihydroxy- 2 - b - glucopyranosyloxy-40-hydroxymethyl benzophenone and 40,60-dicarboxy-20,6-dihydroxy-2-b-glucopyranosyloxy- 40-hy-roxymethyl benzophenone (cassiaphenone A-2-glucoside and cassiaphenone B-2-glucoside, respectively). The naphthalene glycosidetinnevellin- 8-glucosideandkaempferol were also isolated.13
Other constituents present in senna leaves include free sugars (glucose, fructose, sucrose, and pinitol), a mucilage (consisting of galactose, arabinose, rhamnose, and galacturonic acid), and polysaccharides (in C. angustifolia);14,15 flavonoids (isorhamnetin, kaempferol, etc.); a trace of volatile oil; and resins; among others (LIST AND HO¨ RHAMMER). Senna pods normally contain 2–5% sennosides, with Alexandrian pods having higher values than Indian pods. In addition to sennosides A and B, a closely related glucoside, named sennoside A1, has been isolated from Alexandrian senna pods.16 A galactomannon consisting of D-glucose and D-mannose in a 3:2 molar ratio has recently been isolated from the seeds of Indian senna.17
Sennosides are cathartic, with a similar mode of action as cascarosides (see cascara). Sennosides A and C have equal purgative potency in mice but sennoside C has potentiating effects on the activity of sennoside A, exerting a potentiating effect of about 1.6 when 20% of the dose of sennoside A is replaced by sennoside C.18 Senna products along with cascara products are generally considered the drugs of choice among anthraquinone cathartics, and are also generally considered safe (APhA).19,20 Products containing purified sennosides (20 mg) reduced colonic transit time in healthy human volunteers.21 Senna preparations are continuously being evaluated as alternative laxatives in bowel preparation for colonoscopy. In this respect, they have comparative efficacy as sodium phosphate and PEG-electrolyte lavage solutions.22,23
There are controversial data on the adverse effects of senna. On the one hand, it is reported that excessive or prolonged use of senna, as with laxatives in general, may lead to colon damage and other problems (APHA; MARTINDALE).24 Stimulating laxatives should not be used more than 1–2 weeks without medical advice.25 Daily treatment, as with any laxative, is not recommended. Chronic abuse can disturb electrolyte balance, leading to potassium deficiency, heart dysfunction, and muscular weakness, especially under concomitant use of heart-affecting glycosides, thiazide diuretics, corticoadrenal steroids, and licorice root.25Anumber of toxicity cases due to chronic use of senna have recently been reported and include skin breakdown and blisters leading to severe diaper rash in children,26 acute liver failure with renal impairment,27 and subacute cholestatic hepatitis.28 On the other hand, it is reported that senna can be safely administered when given in doses sufficient to produce a motion of physiological water content, even over a long period of time. Senna does not induce specific lesions in the nerve plexus of the intestinal wall, and when used rationally, does not lead to electrolyte losses or habituation. 29 Nonsignificant side effects were observed in rats receiving 750–1500mg/kg/day of senna for 13 weeks. The side effects completely disappeared after 8 weeks of recovery.30 It was also found that daily administration of up to 300 mg/kg/day of senna for 2 years was not carcinogenic to mice.31 Earlier studies showed that senna extracts do not promote malignant tumors in rat colons at laxative doses and that there was no genotoxic risk associated with the use of senna in animals and humans.32,33 One of the polysaccharides exhibited a significant inhibitory effect against solid sarcoma 180 in CD1 mice.14 An ethanolic extract of a preparation containing C. senna (Senokot_) inhibited the effects of different mutagenic agents (e.g.,benzo[a]pyrene, aflatoxin B1) in the Ames test.34

EFFECTS
Laxative Effects
Senna is an anthranoid-type stimulating laxative. The laxative effect is due to the action of sennosides and their active metabolite, rhein anthrone, in the colon. The laxative effect is realized by inhibition of water and electrolyte absorption from the large intestine, which increases the volume and pressure of the intestinal contents. This will stimulate colon motility resulting in propulsive contractions.  In addition, stimulation of active chloride secretion increases water and electrolyte content of the intestine. These changes in active electrolyte transport are dependent on calcium in the serosal surface (Donowitz, 1984; Yamauchi, 1993). The laxative action of Senna is partially via stimulation of colonic fluid and electrolyte secretion, and this secretion is mediated by stimulation of endogenous prostaglandin E2 formation (Beubler. 1988: Yamauchi, 1993).

CLINICAL TRIALS
Laxative Effects
A randomized, single-blind study evaluated the efficacy of Senna compared to polyethylene glycol (PEG) for mechanical preparation for elective colorectal resection. Five hundred twenty-three patients included in the study were undergoing resection, followed by anastomosis. All patients received 5% providone iodine antiseptic enema before surgery, and ceftriaxone sodium and metronidazole were given at anesthesia induction. Senna was significantly better than PEG with regard to colonic cleanliness and less fecal matter in the colonic lumen. The risk for moderate or large intraoperative fecal soiling was lower with senna and overall clinical tolerance did not differ significantly between the treatment groups. Senna was better tolerated in patients with stenosis. There was no statistical difference between the treatment groups with postoperative infective complications or anastomotic leakage (Valverde. 1999). A prospective randomized trial evaluated the efficacy of the addition of senna to a polethylene glycol electrolyte lavage solution (PEG-ELS). One hundred and twenty patients received either a Senna extract with PEG-ELS or placebo with PEG-ELS before a total colonoscopy. Superiority by physician assessment was seen in the group with Senna. The colon was free of solid debris in 66.7% of patients after PEG-ELS and in 90% after Senna/PEG-ELS administration, which was a significant difference. Patient tolerance was similar in both groups, and significantly less lavage fluid was needed in the Senna/PEG-ELS treatment group (Ziegenhagen, 1991).
A randomized, open, parallel group study was conducted to determine the efficacy of senna compared to lactulose in terminal cancer patients treated with opioids. Ninety-one terminal cancer patients were treated with either senna (starting with 0.4 mL daily) or lactulose (starting with 15 mL daily) for a 27-day period. The main outcome measures were defecation-free intervals of 72 hr, days with defecation, general health status, and treatment cost. Both treatment groups had similar scores for defecation-free intervals and in days with defecation. The final scores for general health status were similar in both groups (Agra, 1998).

PURITY TESTS
Microbiology
The test for Salmonella spp. in Folium Sennae products should be negative. The maximum acceptable limits of other microorganisms are as follows (1618). For preparation of decoction: aerobic bacteria—107/g; moulds and yeast—105/g; Escherichia coli—102/g; other enterobacteria—104/g. Preparations for internal use: aerobic bacteria—105/g; moulds and yeast—104/g; Escherichia coli—0/g; other enterobacteria—103/g.
Foreign organic matter
Not more than 2.0% of stems (1) and not more than 1.0% of other foreign organic matter (1, 4, 8).
Total ash
Not more than 12% (5).
Acid-insoluble ash
Not more than 2.0% (1, 8).
Water-soluble extractive
Not less than 3% (1).
Moisture
Not more than 10% (6).
Pesticide residues
To be established in accordance with national requirements. Normally, the maximum residue limit of aldrin and dieldrin in Folium Sennae is not more than 0.05 mg/kg (18). For other pesticides, see WHO guidelines on quality control methods for medicinal plants (16) and guidelines for predicting dietary intake of pesticide residues (19).
Heavy metals
Recommended lead and cadmium levels are not more than 10 and 0.3mg/kg, respectively, in the final dosage form of the plant material (16).
Radioactive residues
For analysis of strontium-90, iodine-131, caesium-134, caesium-137, and plutonium-239, see WHO guidelines on quality control methods for medicinal plants (16).
Other purity tests
Chemical tests and tests of alcohol-soluble extractive are to be established in accordance with national requirements.

INDICATIONS AND USAGE
• Constipation
Senna is used for constipation and for evacuation of the bowel prior to diagnostic tests of the gastrointestinal and colorectal area.
Indian Medicine: The herb is used for constipation, liver disease, jaundice, splenomegaly, anemia, and typhoid fever.
Note: Stimulating laxatives must not be used over a period of more than 1 to 2 weeks without medical advice.

CONTRAINDICATIONS
The herb is not to be administered in the presence of intestinal obstruction, acute inflammatory intestinal diseases or appendicitis.
PRECAUTIONS AND ADVERSE REACTIONS
General: Spasmodic gastrointestinal complaints can occur as a side effect to the drug's purgative effect or from overdosage. In rare cases, prolonged use may lead to cardiac arrhythmias, nephropathies, edema and accelerated bone deterioration. Senna abuse has also resulted in tetany, aspartylglucosamine excretion, and . hypogammaglobulinemia (Levine, 1981; Malmquist, 1980; Prior, 1978).
Electrolyte Abnormalies: Long-term use leads to loss of electrolytes, in particular potassium ions. As a result of hypokalemia, hyperaldosteronism, albuminuria, hematuria, inhibition of intestinal motility, and muscle weakness may occur. Enhancement of cardioactive glycosides and antiarrythics may also occur with hypokalemia.
Finger Clubbing: Senna abuse has resulted in finger clubbing, which was reversible upon discontinuation of the drug (Levine, 1981; Malmquist, 1980; Prior, 1978; Silk, 1975).
Cathartic Colon: Anatomic alteration of the colon is seen secondary to chronic use with Senna (more than three times weekly for 1 year or longer). The result is a loss of haustral folds, a finding that suggests neuronal injury or damage to colonic longitudinal musculature (Joo, 1998).
Carcinogenesis: Carcinogenic activity in the colon following'" long-term administration of anthracene drugs has not yet been fully clarified. Study findings are controversial regarding the correlation between the administration of anthracene drugs and the frequency of carcinomas in the colon (al- Dakan, 1995; Mereto, 1996).
Melanosis Coli: Prolonged use of Senna may lead to melanosis coli. Precursors of the melanic substance in melanosis coli may be derived from anthranoid laxatives (Benavides, 1997).
Occupational Sensitization: IgE-mediated allergy, asthma, and rhinoconjunctivitis have been reported after occupational exposure to senna products (Helin, 1996, Marks, 1991).
Tissue Damage: Chronic treatment with anthranoids in high doses reduces vasoactive intestinal polypeptide and somatostatin levels in the colon, which may represent damage to the enteric nervous tissue (Tzavella, 1985).
General
Use for more than 2 weeks requires medical attention (21, 31).
Drug interactions
Decreased intestinal transit time may reduce absorption of orally administered drugs (32, 33). The increased loss of potassium may potentiate the effects of cardiotonic glycosides (digitalis, strophanthus). Existing hypokalaemia resulting from longterm laxative abuse can also potentiate the effects of antiarrhythmic drugs, such as quinidine, which affect potassium channels to change sinus rhythm. Simultaneous use with other drugs or herbs which induce hypokalaemia, such as thiazide diuretics, adrenocorticosteroids, or liquorice root, may exacerbate electrolyte imbalance (21, 22).
Drug and laboratory test interactions
Urine discoloration by anthranoid metabolites may lead to false positive test results for urinary urobilinogen, and for estrogens measured by the Kober procedure (32).
Carcinogenesis, mutagenesis, impairment of fertility
No in vivo genotoxic effects have been reported to date (3437). Although chronic abuse of anthranoid-containing laxatives was hypothesized to play a role in colorectal cancer, no causal relationship between anthranoid laxative abuse and colorectal cancer has been demonstrated (3840).
Pregnancy: non-teratogenic effects
Use during pregnancy should be limited to conditions in which changes in diet or fibre laxatives are not effective (41).
Nursing mothers
Use during breast-feeding is not recommended owing to insufficient data on the excretion of metabolites in breast milk (21). Small amounts of active metabolites (rhein) are excreted into breast milk, but a laxative effect in breast-fed babies has not been reported (21).
Paediatric use
Contraindicated for children under 10 years of age (21).
Other precautions
No information available on teratogenic effects in pregnancy.

DRUG INTERACTIONS:
Digitalis Glycosides — With prolonged use or abuse of Senna, loss of potassium may potentiate digitalis toxicity.
Antiarrythmics — Loss of potassium associated with prolonged use of Senna may potentiate arrhythmias when given concomitantly with antiarrhythmic medications.
Estrogen — The serum level of estrogen is decreased when given concomitantly with Senna due to the effect of intestinal transit on the absorption of estrogens (Lewis,1998).
Indomethacin (NSAIDS) — Indomethacin given concomitantly with Senna pod extract had a dose-dependent inhibition of net fluid transport due to~ the v inhibition of prostaglandin E2 (SEE EFFECTS), which decreases the therapeutic effect of the Senna (Beubler, 1985).
Nifedipine (calcium channel blocker) — Therapeutic effects induced by rhein anthrone also involve the calcium channel which can be blocked by nifedipine, but not verapamil (SEE EFFECTS) (Yamauchi, 1993).
Pregnancy: The drug should not be used during pregnancy or while nursing.
Pediatric Use: Not to be used by children under 2 years of age. Children between the ages of 2-12 years should follow proper dosage recommendations.
Elderly: Elderly patients should initially take half of the normal prescribing dose.

ADVERSE REACTIONS
Senna may cause mild abdominal discomfort such as colic or cramps (21, 22,33). A single case of hepatitis has been described after chronic abuse (42). Melanosis coli, a condition which is characterized by pigment-loaded macrophages within the submucosa, may occur after long-term use. This condition is clinically harmless and disappears with cessation of treatment (33, 43, 44). Long-term laxative abuse may lead to electrolyte disturbances (hypokalaemia, hypocalcaemia), metabolic acidosis or alkalosis, malabsorption, weight loss, albuminuria, and haematuria (21, 22, 33). Weakness and orthostatic hypotension may be exacerbated in elderly patients when stimulant laxatives are repeatedly used (21, 33). Conflicting data exist on other toxic effects such as intestinal-neuronal damage due to long-term misuse (4554).

DOSAGE
Mode of Administration: Comminuted herb, powder or dried extracts for teas, decoctions, cold macerates, or elixirs. Liquid or solid forms of medication exclusively for oral use.
How Supplied:
·         Capsule — 25 mg, 450 mg
·         Chewable tablet — 15 mg sennosides
·         Granules — 15 mg sennosides per teaspoon
·         Liquid — 2.5 oz. (alcohol 7% by volume), 8.8 mg sennosides per teaspoon
·         Tablet — 8.6 mg sennosides, 15 mg sennosides, 17 mg sennosides, 25 mg sennosides
Preparation: To prepare an infusion, pour hot water (not boiling) over 0.5 to 2 gm of comminuted drug, steep for 10 minutes, then strain; or steep in cold water for 10 to 12 hours, then strain. The cold water method, according to various authors, should result in a solution containing less resin, which is responsible for abdominal pain. The drug takes effect after a latency period of 10 to 12 hours.

PREPARATION FOR SURGERY
• Adult PO black draught: dissolve 3⁄4 oz in 2.5 oz liquid; take between 2 and 4 PM the day before the procedure
Other
·         Adult PO cold infusion, comminuted herb: pour cold water over 0.1-0.2 g herb, let stand 10 hr, strain; 1 _ dose
·         Adult PO granules: add 1⁄2-4 tsp granules to water or juice
·         Adult PO infusion, comminuted herb: pour hot water over 0.1-0.2 g herb, let stand 10 min, strain; 1 _ dose
·         Adult suppositories: insert 1-2 suppositories at bedtime
·         Adult PO syrup: 1-4 tsp at bedtime (7.5-15 ml)
·         Adult PO tablets (Senokot): 1-8 tabs/day
·         Child PO syrup _27 kg: use 1⁄2 adult dose
·         Child PO syrup 1 mo-1 yr: use 1.25-2.5 ml Senokot at bedtime
NOTE: Do not give black draught to children.

DAILY DOSAGE
·         Constipation — The average dose is 20 to 60 mg sennosides.
·         Chewable Tab — Adults and children 12 years of age and over, chew 2 tabs once or twice daily. Children 6 to under 12 years of age, chew 1 tab once or twice daily (Prod Info Ex- Lax®, 1998).
·         Granules (15 mg sennosides per teaspoon) — Adults and children 12 years of age, administer 1 teaspoon once daily with a maximum of 2 teaspoons twice daily. Children 6 to 12 years of age, administer xh teaspoon once daily with amaximum of 1 teaspoon twice a day. Children 2 to 6 years of age, administer lU teaspoon daily with a maximum of lh teaspoon twice daily (Prod Info Senokot®, 1993).
·         Liquid (8.8 mg sennoside per teaspoon) — Children 6 to 12 years of age, administer 1 to 1 'h teaspoon once daily witii a maximum of 1 xh teaspoon twice daily. Children 2 to 6 years of age, administer lh to 3A teaspoon once daily with a maximum of 3A teaspoon twice daily (Prod Info Senokot®, 1991).
·         Pills — Adults and children 12 years of age and over should take 2 pills once or twice daily with a glass of water.
·         Children 6 to under 12 years of age: take 1 pill once or twice daily with a glass of water. Children under 6 years of age: consult a doctor (Prod Info Ex-Lax®, 1998).
·         Tablets (8.6 mg sennosides) — Adults and children 12 years of age, administer 2 tablets once daily with a maximum of 4 tablets twice daily. Children 6 to 12 years of age, administer 1 tablet once daily, with a maximum of 2 tablets twice daily.
·         Children 2 to 6 years of age, adminster xh tablet once daily with a maximum of 1 tablet twice daily (Prod Info Senokot®, 1993).
·         Tablets (17 mg sennosides) — Adults and children 12 years of age, administer 1 tablet once daily with a maximum of 2 tablets twice daily. Children 6 to 12 years of age, administer xh tablet once daily with a maximum of 1 tablet twice daily (Prod Info SenokotXTRA®, 1993).

DOSAGES
 0.5–2 g (0.5–1 tsp)/cup water (APA); 1–2 g fruit (WHO); 3–6 alexandrian or 4–12 tinnevelly pods steeped in 150 ml warm water 6–12 hours (CAN); 0.5–2 g dry leaflets (CAN); 1–2 g dry leaf (PED; WHO); 1 g dry leaf:5 ml alcohol/5 ml water (PED); 0.5–2 ml liquid leaf extract (1:1 in 25% ethanol) (CAN); 20–30 mg hydroxyanthracene derivatives/day calculated as sennoside B (KOM); 2 (25 mg) StX extracts 1–2 ×/day (APA); 10–60 mg sennosides (SKY).

POSOLOGY
The correct individual dose is the smallest required to produce a comfortable, soft-formed motion (21). Powder: 1–2g of leaf daily at bedtime (11). Adults and children over 10 years: standardized daily dose equivalent to 10–30mg sennosides (calculated as sennoside B) taken at night.

Bowel Evacuation:
Liquid (alcohol 7% by volume) — Adults and children 12 years of age and older should take one bottle between 2 and 4 p.m. on day prior to x-ray or other diagnostic procedures. Drink entire contents of bottle. A strong bowel action can be
expected approximately 6 hours after drinking the preparation (Prod Info X-Prep®,1998).
Storage: Senna should be protected from light (DAB 10 EUR), and stored for a maximum of 3 years (2.AB-DDR).
CONTRAINDICATIONS
Pregnancy category is 1; breastfeeding category is 3A.
Senna should not be given to children younger than 12 years of age unless prescribed by a physician. It should not be used by persons with intestinal obstruction, ulcerative colitis, gastrointestinal bleeding, appendicitis, nausea, vomiting, congestive heart failure, or an acute condition in the abdomen caused by surgery. Persons with hypersensitivity to senna should not use it. This herb should not be used for longer than 1-2 weeks without medical advice.

SIDE EFFECTS/ADVERSE REACTIONS
GI: Nausea, vomiting, anorexia, cramping, diarrhea, fl atulence, acute liver failure (senna abuse) (Vanderperren et al, 2005)
GU: Pink, red, brown, or black urine; renal impairment (senna abuse)
INTEG: Hypersensitivity reactions
META: Hypocalcemia, enteropathy, alkalosis, hypokalemia, tetany

INTERACTIONS
Drug
Cardiac glycosides (digoxin): Chronic use of senna may potentiate cardiac glycosides
Disulfi ram: Do not use senna with disulfi ram (Antabuse).
Interactions—cont’d
Laxatives: Avoid the concurrent use of senna with other laxatives; additive effect can occur.
Herb
Jimsonweed: The action of jimsonweed is increased in cases of chronic use or abuse of senna.
Stimulant laxative herbs: Senna may increase the laxative effect of stimulant laxative herbs.
Lab Test
Serum, 24-hour urine estriol: Senna may cause decreased serum and 24-hour urine estriol.
Pharmacology
Pharmacokinetics
Onset of action 6 to 24 hours; metabolized by the liver; excreted in the feces.

CLIENT CONSIDERATIONS
Assess
• Assess for hypersensitivity reactions. If present, discontinue the use of senna and administer an antihistamine or other appropriate therapy.
• Assess stools for color, consistency, character, and presence of blood and mucus.
• Monitor blood and urine electrolytes if the client is using this product often.
• Determine the cause of constipation (e.g., fl uids, bulk, and/or exercise missing from lifestyle).
• Assess for cramping, rectal bleeding, nausea, and vomiting. If these are present, discontinue the use of senna.
• Assess medication and herb use (see Interactions).
Administer
• Instruct the client to store senna products in a sealed container away from heat and moisture.
• Instruct the client to dissolve granules in water or juice before use.
• Instruct the client to shake oral solution before use.
Teach Client/Family
• Inform the client that pregnancy category is 1 and breastfeeding category is 3A.
• Caution the client not to give senna to children younger than 12 years of age.
• Advise the client that the use of laxatives on a regular basis leads to loss of bowel tone.
• Advise the client that urine and feces may turn yellow, brown, or red.
• Advise the client not to use senna if abdominal pain, nausea, or vomiting are present.
Cassia obtusifolia 


Cassiae semen
Jue ming zi

Family: Caesalpinaceae
Standard species: Cassia obtusifolia, C. tora
German: Cassiae-Samen, Sicklepodsamen
English: cassia seeds, foetid cassia seeds, ‘realized brightness seed’

Temperature
 cold tendency

Taste
 bitter
 sweet

Organ relationship
 Liver
 Gall bladder
 Kidney

Direction of action
 sinking

Site of action
Head and eyes

Actions/Indications
 Cools heat
Red and swollen eyes

 Drains fire
Red, painful, light-sensitive eyes, lacrimation, glaucoma, dizziness, vertigo

 Brightens the eyes
Blurred vision, red, swollen, light-sensitive eyes, lacrimation

 Expels wind-heat
Red, painful, itchy, light and wind-sensitive eyes, lacrimation

 Descends the yang
Rectal bleeding due to heat, haematemesis

 Unblocks the bowels/laxative
Constipation due to Liver yin deficiency

PHYSIOLOGICAL/PHARMACOLOGICAL EFFECTS
arteriosclerosis, elevated serum cholesterol (use high dosages 30 g), antibiotic, antihypertensive, diuretic (use with care in combination with other diuretics), increases uterine contractions.

DOSAGE
10–30 g, standard 10 g

COOKING TIME
20 minutes


COMBINATIONS
·         Liver fire with red, painful and swollen eyes, lacrimation, glaucoma; Kidney
·         deficiency Astragali semen (sha yuan ji li) p. 732, Ligustri lucidi fructus (nü zhen
·         zi) p. 794, Lycii fructus (gou qi zi) p. 800, Rehmanniae radix (sheng di huang) p. 168
·         Liver fire or wind-heat with red and painful eyes Chrysanthemi flos (ju hua)
·         p. 52, Mori folium (sang ye) p. 62
·         Liver heat or fire with eye problems Prunellae spica (xia ku cao) p. 140,
·         Gardeniae fructus (zhi zi) p. 126
·         Temporal headache due to wind-heat Ligustici sinensis radix (gao ben) p. 32,
·         Viticis fructus (man jing zi) p. 72
·         Dryness in the Intestines, constipation Angelicae sinensis radix (dang gui) p. 768
·         Constipation with foetor, red eyes, scanty red urine Bambusae folium
·         (xian zhu ye)

COMPATIBILITY
·         Impaired by Cannabis semen (huo ma ren) p. 96
·         Reduces the toxicity of Cinnabaris (zhu sha) p. 418

CONTRAINDICATION
diarrhoea due to Spleen deficiency

PREGNANCY
use with caution


--------------------------------------------‘’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’--------------------------------------------


Cassia grandis L. f. ++


PINK SHOWER
CAESALPINIACEAE


SYNONYMS
Bactyrilobium grande (L. f.) Horn.; B. molle (Vahl) Schrad.; Cassia brasiliana Lam.; C. mollis Vahl; C. pachycarpa Lam.; Cathartocarpus brasilianus (Lam.) Jacq.; Cathartocarpus grandis (L. f.) Pers.; fide (MPG; USN).
Notes:
Thai traditional medicines treat gastrointestinal ailments with C. grandis which may partly explain the lower incidence of gastric cancer in Thailand (X14758718).

COMMON NAMES
Appleblossom Cassia (Eng.; USN); Arbol de Fuego (Sp.; USN); Bacul (Ma.; TTS); Baton Casse (Haiti; AHL; AVP); Beef Feed (Bel.; Eng.; BNA); Bocot (Ma.; JFM); Boocoot (Ma.; JFM); Bookut (Bel.; BNA); Bucut (Guat.; Maya; MPG); Buk-et (Bel.; BNA); Canadonga (Col.; AVP); Canafistola (Brazil; Ven.; AVP); Canafistula (Mex.; Pan.; AVP); Canafistula Burrero (Ma.; Ven.; JFM; LWW); Canafistula Cimarrona (Dor.; Pr.; AHL); Canafistula de Castilla (Ma.; TTS); Canafistula Grande (Ma.; JFM); Canafistula Gruesa (Col.; Ma.; JFM; TTS); Canafistula Macho (Ma.; Ven.; JFM; LWW); Canaflote (Ven.; AVP); Canandonga (Cuba; Ma.; JFM; RyM); Canandonga de Masa (Ma.; JFM); Carago (Sal.; AVP); Caragua (Sal.; AVP); Carague (Guat.; Sal.; AVP; MPG); Caramano (Nic.; AVP); Carambano (Nic.; AVP); Carao (Bel.; Guat.; Hon.; Pan.; AVP; BNA; MPG); Casia (Sp.; AVP); Casse (Haiti; AHL; AVP); Casse de Bresil (Fr.; USN); Casse Espagnol (Haiti; AHL; AVP); Cassia (It.; AVP); Chacara (Dor.; AHL); Chacaro (Dor.; AHL); Coral Shower (Eng.; USN); Coral Showertree (Eng.; VOD); Geneuna (Por.; AVP); Giganton (Ma.; JFM); Great Cassia (Ma.; JFM); Grobfruchtige Kassie (Ger.; USN); Guayaba Cimarrona (Dor.; AHL); Horse Cassia (Eng.; Jam.; AVP; VOD); Jeneuna (Por.; AVP); Kas (Creole; Haiti; VOD); Kas Mawon (Creole; Haiti; VOD); Liquorice Tree (Eng.; TTS); Macut (Ma.; TTS); Maremare (Ma.; JFM); Marimari (Brazil; Peru; AVP; EGG; RAR); Marimary Preto (Ma.; JFM); Marimary Rana (Brazil; Ma.; JFM; LWW); Marimary Sano (Brazil; Ma.; JFM; LWW); Mucut (Guat.; JFM; MPG); Pink Shower (Eng.; FAC; JFM; USN); Pink Showertree (Eng.; VOD); Quauhuayo (Ma.; Mex.; JFM; LWW); Sandal (Ma.; JFM); Sandalo (Ma.; JFM; TTS; USN); Santal (Guat.; MPG); Saragundin (Cr.; IED); Sene Gran’ Fey (Creole; Haiti; VOD); Stinking Toe (Bel.; AVP; BNA; FAC). (Nscn; American entries diacritically prepared).

ACTIVITIES
Abortifacient (f; MPG; VOD); Anticancer (f1; MPG; X14758718); Antiseptic (f; MPG); Antitumor
(f; MPG); Astringent (f; EGG; MPG); Depurative (f; MPG); Diuretic (f; MPG); Expectorant (f; MPG); Febrifuge (f; MPG); Fungicide (1; MPG; X2056755; X8145577); Lactagogue (f; MPG); Laxative (f; MPB; TTS); Pectoral (f; MPG); Purgative (f; AHL; MPG); Sedative (f; MPG); Stimulant (f; MPG); Tonic (f; MPG).

INDICATIONS
Anemia (f; MPG); Arthrosis (f; VOD); Bleeding (f; MPG); Cancer (f1; MPG; X14758718); Colds (f; MPG); Constipation (f1; IED; MPB; TTS); Coughs (f; MPG); Dermatophyte (1; MPG; X2056755; X8145577); Dermatosis (f1; AHL; IED; MPB; MPG; VOD); Dysmenorrhea (f; VOD); Dyspepsia (f; VOD); Enterosis (f; IED; VOD); Epistaxis (f; MPG); Fever (f; MPG); Fungus (f1; MPG; X2056755; X8145577); Gastrosis (f; VOD); Hepatosis (f; MPG); Herpes (f; MPG); Hysteria (f; VOD); Infection (f1; MPG; VOD); Insomnia (f; MPG); Itch (f; VOD); Mange (f; MPG); Mucososis (f; MPG); Mycosis (f; MPG); Nervousness (f; VOD); Parasites (f; IED); Pulmonosis (f; IED); Respirosis (f; IED); Rheumatism (f; VOD); Sores (f; IED); Tinea (f; MPG); Urethrosis (f; MPG); Viruses (f; MPG); Vitiligo (f; MPG); Worms (f; IED); Wounds (f; MPG).

DOSAGES
FNFF = ! Pulp around seeds edible (IED; FAC; MPG).
Costa Ricans use the fruit pulp, cooked i • n milk, for anemia (JFM).
·         Cubans consider the fruit pulp abortifacient and useful in chest complaints (JFM).
·         Cubans steep roots 3 days in alcohol as antiseptic for dermatosis and wounds (RyM).
·         Dominicans suggest mashed leaves with bacon fat for veterinary skin ailments (AHL).
·         Guatemalans fashion an unguent from the leaves for dermatosis, herpes, sores, tinea, and vitiligo (MPG).
·         Guatemalans take bark/fruit/leaf decoction for anemia, cold, cough, hepatosis, hysteria, nosebleed, and urinary infections (MPG).
·         Haitians apply macerated root tincture for skin infections (VOD).
·         Haitians massage skin ailments, like itch, with crushed leaves (VOD).
·         Haitians drink a beverage made from leaf, flower, fruit pulp, a/o seeds as an abortifacient and for hysteria and nervousness (VOD).
·         Haitians take root and bark infusion for rheumatism (VOD).
·         Haitians take salted leaf decoction for digestive tract ailments (VOD).
·         Nicaraguans use fruit and leaf decoction, or juice syrup, orally or topically for constipation, respiratory-pulmonary disorders, worms and intestinal parasites, skin rashes, and sores (IED).

DOWNSIDES
Too much of the edible fruit pulp is said to be abortifacient and certainly laxative. As of July 2007, the FDA Poisonous Plant Database listed four titles alluding to toxicity of this species.

EXTRACTS
Methanolic leaf extract inhibited growth of HP strains MIC = 50.0 μg/ml.

REFERENCE

Duke, J. A. with Mary Jo Bogenschutz-Godwin, Judi duCellier, Peggy-Ann K. Duke. 2002. Handbook of Medicinal Herbs 2nd Ed. CRC Press LLC. USA. 

Duke, J. A With Mary Jo Bogenschutz-Godwin Andrea R. Ottesen. 2009. Duke’s Handbook of MEDICINAL PLANTS OF LATIN AMERICA.CRC Press Taylor & Francis Group, LLC. USA

Hempen, C-H and Fischer, T. 2009. A MATERIA MEDICA FOR CHINESE MEDICINE Plants, Minerals and Animal Products. Elsevier Limited. German.

IKHLAS A. KHAN AND EHAB A. ABOURASHED. 2010. LEUNG’S ENCYCLOPEDIA OF COMMON NATURAL INGREDIENTS USED IN FOOD, DRUGS, AND COSMETICS Third Edition. John Wiley & Sons, Inc. Hoboken, New Jersey

Jing-Nuan Wu. 2005. A N I L L U S T R A T E D Chinese Materia Medica. Oxford University Press, Inc. New York.

Linda S-R. 2010. MOSBY’S HANDBOOK OF HERBS & NATURAL SUPPLEMENTS, FOURTH EDITION. Mosby, Inc., an affi liate of Elsevier, Inc. USA.   

Ling, K H., Kian, C T ., and Hoon, C T. 2009.  A GUIDE TO MEDICINAL PLANTS An Illustrated, Scientific and Medicinal Approach. World Scientific Publishing Co. Pte. Ltd. Singapore

Medical Economics Company, Inc. 2000. PDR for Herbal Medicines". Medical Economics Company, Inc. at Montvale, NJ 07645-1742.

Ross, I. A. 2003Medicinal Plants of the World Vol. 1. Chemical Constituents, Traditional and Modern Medical Uses. Human Press. Totowa, New Jersey.

Simon, D and Chopra, D. 2000. THE CHOPRA CENTER HERBAL HANDBOOK Natural Prescriptions for Perfect Health. Three Rivers Press, New York.

THE MINISTER OF HEALTH OF THE REPUBLIC OF INDONESIA 2016. REGULATION OF THE MINISTER OF HEALTH OF THE REPUBLIC OF INDONESIA NUMBER 6 OF 2016 REGARDING THE ORIGINAL HERBAL MEDICINE FORMULARIUM OF INDONESIA. Jakarta. Indonesia.  

Tierra, M and Tierra, L. 1998. Chinese Traditional Herbal Medicine Vol 2. Materia Medica and Herbal Resource. Twin Lakes, WI. USA.

World Health Organization (WHO). 1999. WHO Monographs on Selected Medicinal Plants Volume 1. Geneva. Swiss.













No comments:

Post a Comment